Save antibodies
 

To use less than 2 ml of diluted antibodies per blot pack the blot tightly into a sealy-bag, and add your antibodies onto the protein side. 

Worried about little air bubbles in your sealy-bag now?

1) Tape it face-up onto a container.

2) Put balls (squash, golf) onto this container.

3) Put the container onto a shaker to shake the balls around.  These will keep your antibody solution and any air bubbles inside the sealy-bag moving around throughout your incubation period.

Western blotting
   

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