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Issue 13, June 2009
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Laboratory Feng Shui How NOT to write your cover letter Its too hot in the lab when... Changing that light bulb... Do you get it? My mother taught me: Some of the latest and most accurate star signs Famous last words What do you know about referees? (For publishing your paper!) Quotes that could make a good T-shirt to wear in your lab You know you've had a BAD week of experiments when: How smart are you? More from Improbable Research You know you've been in the lab too long when... Why graduate students are like cats You might be a scientist if you: Why study? Emergency room Some 2008 IGŪ Nobel Prize winners A key to scientific literature A short history of medicine How time is spent 9 rules for research 3 more rules to research Teaching maths
| Heated your solution and you need to spin it back down? Working with multi-well plates Are you filtering your solution through a funnel? Cleaning with alcohol? Storing forceps and tweezers Wash bottles faster Get your money's worth! Skip stirring No leaks Don't lose samples from evaporation Opening microtubes Avoid bubbles in your plates Save cost Permanent markers | Storing FBS long term Judging your FBS quality
Spray ethanol earlier Getting regular contamination with bacteria? How to keep your cells warm out of the hood Warm your media in aliquots Culture media DMSO + media is exothermic No background staining for fluorescence Counting cells The safer side Are you growing your cells in little petri dishes? Can't find your cell freezing container? Seeding cells into a multiwell plate Using adherent cells? What's a common source of contamination? An easy way to visualise your cell's morphology
| Increase aeration No air bubbles when pouring plates Storing media
| Feeling nauseous when using your microscope? Are there spots of poorer focus on your microscopy images? Clean lenses Ditch lens tissues | Combine your protein ladders Load more sample Marking out your blot Save antibodies
Slow down! | How do you reduce aggressive behaviour? Intraperitoneal injections - hit the spot each time Keeping your animal warm Permanent labels on animals Reduce pain at the injection site Subcutaneous injections - hit the spot each time Withdrawing blood from tails | Handling DNA Handling RNA
| Increase accuracy by pre-wetting your tip Have you found that some of your 'sterile' pipette tips are dirty? Are you pipetting small volumes from your bottle or centrifuge tube? Have you been pipetteing volatile liquids? Increase accuracy with viscous samples Pipette viscous liquids easier Serological pipettes: how you stop the dribbling! Pre-wet your pipette to increase accuracy | Last minute dissertation writing Dissertation writing
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