Don't lose samples from evaporation
 

When using a 96-well plate that needs to go into an incubator (such as for cell culture), it’s better to only use it's inner wells.  The outer wells lose sample over time in an incubator (and corner wells lose the most).

Consider using a plate map like below, where you only use 60 wells at most, and fill the outer wells with some liquid to buffer sample loss (e.g. PBS)

Plate map

   

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