Avoid bubbles in your plates
 

Use reverse pipetting, especially for 96-well plates for assays so you won’t get bubbles into the well.  This is particularly important for assays with a plate reader.

Use a centrifugeBut if you do get bubbles, remove them easily in 1-2 mins by spinning the plate at 4-5000 rpm… unless you want to pop the bubbles individually!

   

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