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When passaging cells, standard protocol tells you to wash your suspended cells with media to remove remaining drops of trypsin, then to spin the cells into a pellet and pour off the supernatant. And then you resuspend this pellet to count your cells with a hemocytometer. ...Why don't you try taking a sample of cells out first before spinning your cells into a pellet and do a cell count then? This way you can already work out the number of cells you have before resuspending the pellet.
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