DMSO + media is exothermic
 
DMSO is often added to media to help freeze down cells.
Heat
But be careful!  Adding DMSO to media creates an exothermic reaction, heating up your media.
If you pre-heat your media to 37°C, your media could heat up to 40°C.  Remember that your cells are less tolerant to heat shocks than to cold shocks, so this could kill some of your cells even before they start getting frozen down!
So if you tend to pre-heat your media, try to offset this by giving it time to equilibrate back to 37°C.
   

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